目的探讨1,25-二羟维生素D3(1, 25(OH)2D3)对IgA 肾病大鼠Th1 细胞(IFN-γ)的影响。方法将造模成功的32 只IgA 肾病模型大鼠随机分为强的松治疗组、1,25(OH)2D3治疗组、强的松+1, 25(OH)2D3联合治疗组及未干预组，同时设立正常对照组。检测各组大鼠24h 尿蛋白、血肌酐及IFN-γ的水平。结果①A 组(正常对照组)、B 组(未干预组)、C 组(强的松治疗组)、D 组(1，25(0H)2D3治疗组)及E 组(强的松+1，25(0H)2D3联合治疗组)的24h 尿蛋白分别是7.03±0.99mg/d、51.49± 3.04 mg/d、12.15±0.75mg/d、23.0±2.27mg/d 及9.99±0.79mg/d，其中A 组、C 组、D 组及E 组的尿蛋白水平较B 组下降，差别有统计学意义(F=47.778，P＜0.05)。②A 组、B 组、C 组、D 组及E 组的脾脏IFN-γ水平分别是0.64%±0.05%、3.63%±0.22%、2.11%±0.13%、1.33%±0.10%及0.97%±0.17%，相应的淋巴结IFN-γ水平分别是2.13%±0.42%、17.32%±0.65%、8.44%±0.38%、6.39%±0.57%及3.01%±0.27%，其中A 组、C 组、D 组及E 组的IFN-γ水平较B 组显著下降，差别有统计学意义(F =479.381，P＜0.05)。结论1,25(OH)2D3可降低IgA 肾病大鼠尿蛋白及IFN-γ的水平。

Objective Explore the mechanism of 1α,25-dihydroxyvitaminD3 regulation on Th1 cells in IgA nephropathy rats. Method The model of 32 IgA nephropathy rats were randomly divided into prednisone treatment group, 1α,25- dihydroxyvitaminD3 treatment group,prednisone + 1α,25- dihydroxyvitaminD3 treatment group and non treatment group.At the same time,we set up the normal control group.24 hours urine protein,serum creatinine value and IFN- γwere measured in each group. Results ①The level of 24-hour urine protein in group A,B,C,D and E, are respectively 7.03±0.99mg/d,51.49±3.04mg/d,12.15±0.75mg/d,23.0± 2.27mg/d and 9.99±0.79mg/d, in which the group A,C,D and E decreased significantly compared with the group B (F=47.778, P<0.05). ②The level of IFN-γin spleen of group A,B,C,D and E are respectively 0.64%± 0.05%,3.63%±0.22%,2.11%±0.13%,1.33%±0.10% and 0.97%±0.17%.The level of IFN-γin Lymph Node of
group A,B,C,D and E are respectively 2.13%± 0.42%,17.32%± 0.65%,8.44%± 0.38%,6.39%± 0.57% and 3.01%±0.27%, among the group A,C,D and E decreased significantly compared with the group B(F=479.381，P＜0.05). Conclusion 1α,25-dihydroxyvitaminD3 can reduce the level of urinary protein and IFN-γ in IgA nephropathy rats.