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临床研究

Irisin 与维持性血液透析患者肌肉减少症的相关性研究

  • 王亚美 ,
  • 刘风勋 ,
  • 邢利 ,
  • 程艳 ,
  • 张靖华 ,
  • 梁献慧 ,
  • 王沛 ,
  • 刘章锁
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  • 1. 郑州大学第一附属医院血液净化中心
    2. 郑州市人民医院肾内科
    3. 郑州市第三人民医院肾内科
    4. 郑州市第七人民医院肾内科

收稿日期: 2019-04-01

  修回日期: 2019-07-23

  网络出版日期: 2019-09-27

The correlation between irisin and sarcopenia in maintenance hemodialysis patients

  • WANG Ya-Mei ,
  • LIU Feng-Xun ,
  • XING Li ,
  • CHENG Yan ,
  • ZHANG Jing-Hua ,
  • LIANG Xian-Hui ,
  • WANG Pei ,
  • LIU Zhang-Suo
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  • 1Blood Purification Center, the First Hospital Affiliated to Zhengzhou University, Zhengzhou 450052;  2Department of Nephrology, Zhengzhou Municipal Hospital, Zhengzhou 450003;  3Department of Nephrology, the Third Peoples Hospital of Zhengzhou, Zhengzhou 450001;  4Department of Nephrology, the Seventh Peoples Hospital of Zhengzhou, Zhengzhou 450006, China

Received date: 2019-04-01

  Revised date: 2019-07-23

  Online published: 2019-09-27

摘要

【摘要】目的探究血清Irisin 水平与维持性血液透析(maintainence hemodialysis,MHD)患者生存率的关系及Irisin 对成肌细胞自噬的影响,为Irisin 治疗肌肉减少症提供证据。方法纳入180 例MHD 患者,根据亚洲肌肉减少症工作组(asia working groups for sarcopenia, AWGS)制定的肌肉减少症的诊断共识,分为肌肉减少症组与非肌肉减少症组,用ELISA 法检测MHD 患者血清中Irisin 的浓度,分析Irisin 水平与MHD 患者生存率的关系;Western Blot 检测硫酸吲哚酚(indoxyl sulfate,IS)诱导的成肌细胞自噬相关蛋白的表达,分析Irisin 对成肌细胞自噬的影响。结果在MHD 患者中肌肉减少症组血清Irisin 水平明显低于非肌肉减少症组(t=-4.802,P<0.001),经过3 年的随访,低Irisin 水平组患者的死亡率高于高Irisin 水平组患者。自噬蛋白Atg-3,Atg-12 和Beclin-1 在IS 组表达增加,随着Irisin 的浓度的增加抑制了Atg-3,Atg-12 和Beclin-1 的表达。结论低Irisin 水平的MHD 患者死亡率高;Irisin 改善IS 诱导的成肌细胞自噬。

本文引用格式

王亚美 , 刘风勋 , 邢利 , 程艳 , 张靖华 , 梁献慧 , 王沛 , 刘章锁 . Irisin 与维持性血液透析患者肌肉减少症的相关性研究[J]. 中国血液净化, 2019 , 18(10) : 677 -680 . DOI: 10.3969/j.issn.1671-4091.2019.10.005

Abstract

【Abstract】Objective The aim of this study was to determine the relationship between serum Irisin level and survival rate in maintenance hemodialysis (MHD) patients and the effect of irisin on autophagy in myoblasts. This study was expected to provide an evidence of irisin useful for the treatment of sarcopenia. Design and Method A total of 180 MHD were recruited in this study. Based on the common recognition about the diagnosis of sarcopenia from Asia Working Groups for Sarcopenia (AWGS) in 2014, patients were divided into sarcopenia group and non-sarcopenia group. Serum irisin was determined by ELISA, and the relationship between serum Irisin and survival rate of the patients was then analyzed. The expression of autophagy related genes in murine C2C12 myoblasts induced with indoxyl sulfate (IS) were assayed by western blot, and the effect of Irisin on autophagy in myoblasts was then evaluated. Results Serum irisin was significantly lower in sarcopenia group than in non-sarcopenia group (t=-4.802, P<0.001). Mortality rate was higher in patients with lower serum Irisin than in those with higher serum irisin after the follow-up study for 3 years (χ2=7.865, P=0.010). The autophage related proteins of Atg-3, Atg-12 and Beclin-1 increased significantly in IS induced C2C12 myoblasts, and these changes could be ameliorated by the elevation of Irisin concentration. Conclusions The mortality rate was higher in MHD patients with lower serum Irisin level. Irisin ameliorated the autopahge in IS induced myoblasts.

参考文献

[1]Cruz-Jentoft A J, Baeyens J P, Bauer J M, et al.Sarcopenia: European consensus on definition and diagnosis: Report of the european working group on sarcopenia in older people[J].Age and ageing, 2010, 39(4):412-423
[2]任红旗,龚德华.尿毒症肌少症的发病机制和治疗[J].肾脏病与透析肾移植杂志, 2015, 24(2):181-185
[3]Landi F, Calvani R, Cesari M, et al.Sarcopenia: An overview on current definitions,diagnosis and treatment[J].Current protein & peptide science, 2018, 19(7):633-638
[4]Enoki Y, Watanabe H, Arake R, et al.Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1[J].Scientific reports, 2016, 6:32084-
[5]Enoki Y, Watanabe H, Arake R, et al.Potential therapeutic interventions for chronic kidney disease-associated sarcopenia via indoxyl sulfate-induced mitochondrial dysfunction[J].J Cachexia Sarcopeni, 2017, 8(5):735-747
[6]Pedersen B K, Febbraio M A.Muscles,exercise and obesity: Skeletal muscle as a secretory organ[J].Nat Rev Endocrinol, 2012, 8(8):457-465
[7]Bostrom P, Wu J, Jedrychowski M P, et al.A pgc1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis[J].Nature, 2012, 481(7382):463-468
[8]Huh J Y.Fndc5 and irisin in humans: I.Predictors of circulating concentrations in serum and plasma and ii. Mrna expression and circulating concentrations in response to weight loss and exercise[J].Metabolism, 2012, 61(12):1725-1738
[9]Reza M M, Subramaniyam N, Sim C M, et al.Irisin is a pro-myogenic factor that induces skeletal muscle hypertrophy and rescues denervation-induced atrophy [J].Nature communications, 2017, 8(1):1104-1114
[10]Rosenberg I H.Sarcopenia: Origins and Clinical Relevance[J].Journal of Nutrition, 2011, 27(3):337-339
[11]Lamarca F, Carrero J J, Rodrigues J C, et al.Prevalence of sarcopenia in elderly maintenance hemodialysis patients: The impact of different diagnostic criteria[J].The journal of nutrition, health & aging, 2014, 18(7):710-717
[12]Frontera W R, Hughes V A, Fielding R A, et al.Aging of skeletal muscle: a 12-yr longitudinal study[J].Journal of Applied Physiology, 2000, 88(4):1321-1326
[13]Fahal, I.H . Uraemic sarcopenia: aetiology and implications[J].Nephrology Dialysis Transplantation, 2014, 29(9):1655-1665
[14]Hyun Y Y, Lee K B, Han S H, et al.Nutritional status in adults with predialysis chronic kidney disease: Know-ckd study[J].Journal of Korean medical science, 2017, 32(2):257-263
[15]Chen N, Karantza-Wadsworth V.Role and regulation of autophagy in cancer[J].Biochim Biophys Acta, 2009, 1793(9):1516-1523
[16]Grumati P, Coletto L, Sabatelli P, et al.Autophagy is defective in collagen vi muscular dystrophies,and its reactivation rescues myofiber degeneration[J].Nat Med, 2010, 16(11):1313-U1169
[17]Sandri M.Autophagy in health and disease3. Involvement of autophagy in muscle atrophy[J].American journal of physiology Cell physiology, 2010, 298(6):C1291-1297
[18]Petrovski G, Das D K.Does autophagy take a front seat in lifespan extension?[J].Journal of Cellular and Molecular Medicine, 2010, 14(11):2543-2551
[19]Levine B, Kroemer G.Autophagy in the Pathogenesis of Disease[J].Cell, 2008, 132(1):0-42
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