【Abstract】Objective To observe the application and nursing of mixed dilution method in hemodiafiltration treatment in plateau area. Methods A total of 76 hemodialysis patients were randomly divided into experiment group (mixed dilution method, n=39) and control group (post-dilution method, n=37). The effects of toxin clearance, coagulation in dialyzer, amount of heparin used, alarm frequency, and the subjective comfort degree were compared between the two groups. Reults Blood samples were taken before and after dialysis, and renal function tests were performed. Compared with post- dilution method, mixed dilution method increased the decreased creatinine clearance rate by 25.47%(t=9.706, P=0.024), the decreased urea nitrogen clearance rate by 12.21% (t=5.571, P=0.047), and the decreased β2 microglobulin clearance rate by 14.41% (t=7.669, P= 0.038). By the observation of coagulation grade in dialyzer and the amount of heparin used, the mixed dilution method also showed the advantages of improving coagulation grade in dialyzer and reducing heparin amount used by 50.63% (from 6.32 IU to 3.12 IU; t=25.469, P=0.010). The subjective comfort degree observed by a suitable scale demonstrated that mixed dilution method increased the quality of life score by 38.66% (t=4.444, P=0.035) and appetite score by 37.28% (t=5.845, P=0.042), and decreased sleep quality score by 50.63% (t=25.469, P=0.028) and skin itching degree score by 44.55% (t=15.132, P=0.036). Conclusion With the prerequisite for adequate effect of hemodiafiltration, mixed dilution method is more suitable than post-dilution method for hemodialysis patients in plateau areas. Therefore, this method of hemodiafiltration is useful for clinical use.