【Abstract】Objective To improve the quality of care in installation and pre-flush for hemodialysis therapy by applying a quality control checklist. Methods A quality control checklist to standardize the installation and pre-flush was designed and used as an evaluation tool to investigate 11 hemodialysis institutions/centers during the priming process. The medical workers in the 11 dialysis centers were then trained according to content in the checklist. The processes of installation and pre-flush of each dialysis unit were evaluated and the efficacy of the training was obtained and discussed after training. Results There was no significant difference in the priming time before and after the quality improvement (27.05 ± 4.59 vs. 27.50 ± 4.03 min; t= -0.296, P=0.774). All of the compliance rates were improved after quality improvement, and the overall score was higher after the training compared to that before the training (t=-3.993, P=0.003). All of the 11 dialysis centers considered that the checklist was quite simple and practical. Conclusion The priming quality control checklist is convenient and practical for training and evaluation. This checklist can be used by the clinical quality managers as a regular evaluation method for the quality control of installation and pre-flush for hemodialysis therapy.