【Abstract】 Objective To observe the effects of lipopolysaccharide (LPS) on the expression of inflammatory factors interleukin 15 (IL-15) and interleukin 6 (IL-6) and malondialdehyde (MDA) in rat peritoneal mesothelial cells (RPMCs). Methods RPMCs were isolated and cultured using trypsin, and then identified. RPMCs at passage 3 were used in the experiment. The cells were divided into (i) normal control group; (ii) LPS dose/time group, LPS 1mg/L, 10mg/L or 100mg/L treated for 6 hours or 12 hours; (iii) LPS time group, LPS 10mg/L treated for 3, 6, 12 and 24 hours. IL-15 mRNA was measured by real-time RT-PCR. IL-15 and IL-6 were assayed by ELISA. MDA were determined by thiobarbituric acid method. Results IL-6, IL-15 and its mRNAs, and MDA were significantly increased in a dose-dependent manner and/or a concentration-dependent manner in the groups treated with LPS (p<0.05, compared with those of the control group). Conclusion IL-15 may be involved in the pathological processes of peritoneal dialysis-related peritonitis. LPS can up-regulate the expression of IL-15, IL-6 and MDA in RPMCs, possibly relating to the peritonitis and oxidative stress.
PENG Xia
,
MA Jian-fei
,
LI Cheng-cheng
,
FAN Yi
,
HUO Ai-jing
. The effects of lipopolysaccharide on the expression of inflammatory factors interleukin 15 and interleukin 6 and malondialdehyde in rat peritoneal mesothelial cells[J]. Chinese Journal of Blood Purification, 2011
, 10(3)
: 149
-153
.
DOI: 10.3969/j.issn.1671-4091.2011.03.00
