Objective The accuracy, effectiveness and safety of the mobile continuous blood purification machine were evaluated clinically in order to address the possibility of clinical applications. Methods The mobile continuous blood purification machine was compared with Baxter Aquarius continuous blood purification machine in their accuracy, effectiveness and safety. Auto-cross design was used in the study. Results The accuracies for dehydration and for replacement flow control of the mobile continuous blood purification machine varied from 0.1-0.59% and from 0.00-1.48%, respectively, consistent with the national standards and comparable to those of Baxter Aquarius continuous blood purification machine (P＞0.05). During the operation of the mobile continuous blood purification machine, the alarm systems worked accurately and sensitively without any mechanical accident, and the vital signs of the subjects were stable. After dialysis with the mobile continuous blood purification machine, patients’ blood creatinine and urea decreased significantly, acidosis and electrolyte imbalances improved greatly, demonstrating the achievements indifferent from Baxter Aquarius continuous blood purification machine (P＞0.05). Conclusions The mobile continuous blood purification machine is accurate, effective, safe and stable in clinical trials, and is worthwhile to be recommended for clinical applications.