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Chinese Journal of Blood Purification >

2010 , Vol. 9 >Issue 8: 436 - 410

DOI: https://doi.org/10.3969/j.issn.1671-4091.2010.08.008

临床研究

The role of reactive oxygen species in the promotion of monocyte chemoattractant protein-1 (MCP-1) production by advanced glycation end products-human serum albumin (AGE-HSA ) in human peritoneal mesothelial cells

  • HONG Fu-yuan ,
  • SUN Fang ,
  • LIU Jun ,
  • YAO Jian ,
  • HUANG Yi-xin ,
  • TANG Zhi-huan
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  • 1Department of Nephrology, Fujian Provincial Hospital, Fuzhuo 350001, China; 2Department of Nephrology, Shanghai First People’s Hospital, Shanghai 200080, China

Received date: 2009-12-03

  Revised date: 1900-01-01

  Online published: 2010-08-12

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Abstract

【Abstract】 Objective To study the effects of advanced glycation end products-human serum albumin (AGE-HSA) on the production of monocyte chemoattractant protein-1 (MCP-1) in human peritoneal mesothelial cells (HPMC) and the role of reactive oxygen species (ROS) in this process. Methods AGE-HSA (0, 100, 500 and 1000μg/mL) with or without 30mM N-acetyl-L-cysteine (NAC) were added to the cell culture medium to stimulate HPMC. MCP-1 mRNA was measured by semi-quantitative RT-PCR, and MCP-1 protein in HPMC was determined by ELISA. The cells were marked with oxidation–susceptible flurorescent probe 2,7-dichorofluoresin diacetate (DCFH) and then were assayed by flow cytometry. Results AGE-HSA increased the concentration of ROS in HPMC with a dose-dependent manner. AGE-HSA also stimulated the production of MCP-1 in dose- and time- dependent manners. The effects of AGE-HSA on HPMC could be blocked by antioxidant NAC. Conclusion The induction of ROS by AGE-HSA in HPMC stimulates the expression of MCP-1. This process may play an important role in the inflammatory reaction and may lead to the ultrafiltration failure.

Key words： Reactive oxygen species; MCP-1; Peritoneal dialysis

Cite this article

HONG Fu-yuan , SUN Fang , LIU Jun , YAO Jian , HUANG Yi-xin , TANG Zhi-huan . The role of reactive oxygen species in the promotion of monocyte chemoattractant protein-1 (MCP-1) production by advanced glycation end products-human serum albumin (AGE-HSA ) in human peritoneal mesothelial cells[J]. Chinese Journal of Blood Purification, 2010 , 9(8) : 436 -410 . DOI: 10.3969/j.issn.1671-4091.2010.08.008

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