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Chinese Journal of Blood Purification >

2010 , Vol. 9 >Issue 9: 498 - 502

DOI: https://doi.org/10.3969/j.issn.1671-4091.2010.09.006

临床研究

Study on the inhibition effect of the short peptide AcSDKP on renal interstitial fibrosis in rats

  • WANG Ming-ao ,
  • LIU Rui-chan ,
  • BAO Yu-shi ,
  • GU Yun-he ,
  • MU Su-hong ,
  • XIE Ru-juan
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  • 1Department of Nephrology, 2Department of Pathology, The First Clinical Hospital Affiliated to Harbin Medical University, 150001, China

Received date: 2010-01-07

  Revised date: 1900-01-01

  Online published: 2010-09-12

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Abstract

【Abstract】Objective To investigate the effect and mechanism of the short peptide acetyl-Ser-Asp-Lys-Pro (AcSDKP) on renal interstitial fibrosis induced by unilateral ureteral obstruction in rats. Methods Thirty-six male Wistar rats were randomly divided into sham-operation group, unilateral ureteral obstruction (UUO) group, and UUO+AcSDKP treatment group. Animal model of rat renal interstitial fibrosis was established by unilateral ureteral ligation. Rats were sacrificed after the surgery for 3 or 14 days. Histological change of the obstructed kidney was observed by H-E staining. The protein expression and localization of transforming growth factor β1 (TGF-β1) and -smooth muscle actin ( -SMA) in renal tissues were detected by immunohistochemistry. The mRNA levels of TGF-β1 and -SMA were measured by reverse transcription- polymerase chain reaction. Results Compared with sham-operated group, rats in UUO group exhibited severe renal interstitial fibrosis and higher expression of TGF-β1 and -SMA (P＜0.05), while those in UUO+AcSDKP group demonstrated less renal interstitial fibrosis, and lower expression levels of TGF-β1 and -SMA (P＜0.05). Conclusions AcSDKP treatment improves renal interstitial fibrosis in rats by down-regulating TGF-β1 and -SMA expression.

Key words： Renal interstitial fibrosis; Transforming growth factor β1

Cite this article

WANG Ming-ao , LIU Rui-chan , BAO Yu-shi , GU Yun-he , MU Su-hong , XIE Ru-juan . Study on the inhibition effect of the short peptide AcSDKP on renal interstitial fibrosis in rats[J]. Chinese Journal of Blood Purification, 2010 , 9(9) : 498 -502 . DOI: 10.3969/j.issn.1671-4091.2010.09.006

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