Objective  To study the application effect of internet-based intelligent gripper to promote maturation of autologous arteriovenous fistula (AVF) in hemodialysis patients.  Methods  A total of 40 patients with end-stage renal disease were recruited and assigned to control group that used traditional grip strength exercise training in The Second Affiliated Hospital of Suzhou University during January 2021 to December 2021; a total of 30 patients using the internet-based intelligent gripper strength training were assigned to research group from January 2022 to December 2022. Maturation rate of the AVF, first puncture time, success rate of the first puncture, puncture complication, and compliance with postoperative grip strength exercise training were compared between the two groups. Results After the surgery for 4 weeks, clinical maturation rate (χ²=1.542, P=0.228) and ultrasound maturation rate (χ²=0.420, P=0.517) had no significant differences between the two groups. After the surgery for 8 weeks, clinical maturation rate (χ²=16.324, P<0.001), ultrasound maturation rate (χ²=12.343, P<0.001), venous diameter (t=8.358, P<0.001), and brachial artery flow   (t=8.320, P<0.001) were higher in research group than in control group, and the depth from vein to skin was lower in research group than in control group (t=12.718, P<0.001). In research group, the first use of the AVF was earlier (t=6.665, P<0.001), the success rate of the first puncture was higher (χ²=13.611, P<0.001), the complication rate was lower (χ²=5.605, P=0.018), and the compliance rate with postoperative grip exercise training was higher (χ²=7.805, P=0.005), as compared those with control group.  Conclusion Internet-based intelligent gripper training can shorten the maturation time of AVF, improve the maturation rate of AVF, ensure success rate of the first puncture, reduce the incidence of complications, and improve the compliance with grip exercise training in hemodialysis patients.